Studies on Polyphenol Oxidase of Tricholoma matsutake(S. Ito et Imai) Sing

송이(松?)버섯 [Tricholoma matsutake(S. Ito et Imai) Sing.]의 Polyphenol Oxidase에 관하여

The characteristics of crude polyphenol oxidase extracted from mushroom[Tricholoma matsutake(S. Ito et Imai) Sing.] were investigated. The enzyme showed highest affinity to pyrogaroll among trihydroxyphenols. Except for o-diphenols the enzyme was inactive in di-and monophenols. The optimum pH was about 4 and the optimum temperature ranged from 45 to $55^{\circ}C$. The enzyme activity was completely inhibited by $70^{\circ}C$ heat treatment for 2 min. $Cu^{++}$, $Fe^{++}$ and $Mg^{++}$ activated the enzyme at low concentration($10^{-1}mM$), but inhibited at high concentration (1mM). The most potent inhibitors were Na-diethyldithiocarbamate, L-ascorbic acid, L-cysteine and NaCl. The Km value with pyrogaroll was 0.88mM.

송이버섯[Tricholoma matsutake(S. Ito et Imai);Sing.]에서 polyphenol oxidase 조효소액(粗酵素液)을 추출(抽出), 그 성질(性質)을 조사(調査)하여 다음과 같은 결과(結果)를 얻었다. Trihydroxyphenol류(類)와 o-diphenol류(類)에 활성(活性)을 보였고 이들중 pyrogaroll이 최고활성(最高活性)을 보였으며 m-diphenol, p-diphenol 그리고 monophenol류(類)에서는 활성(活性)을 보이지 않았다. 최적(最適) pH는 4.0 부근이었고, 최적온도(最適溫度)는 $45{\sim}55^{\circ}C$였으며, 효소(酵素)의 활성(活性)이 $70^{\circ}C$에서 2분(分) 가열(加熱)로 완전(完全)히 실활(失活)되었다. $Cu^{++}$, $Fe^{++}$ 및 $Mg^{++}$은 $10^{-1}mM$이상(以上) 농도(濃度)에서는 효소력(酵素力)이 증가(增加)되었으나, 그 이상(以上)의 농도(濃度)에서는 오히려 저하(低下)되었다. 저해제(沮害劑)로는 Na-DETC, L-ascorbic acid, L-cysteine 및 NaCl이 양호(良好)하였다. pyrogaroll에 대한 km치(値)는 0.88mM이었다.