HPLC를 이용(利用)한 제제중(製劑中) 프레드니솔론의 함량균일성시험(含量均一性試驗)에 관한 연구(硏究)

Determination of Prednisolone in Tablets by High Performance Liquid Chromatography

A convenient high performance liquid chromatographic method for the quantitative determination and content uniformity of prednisolone in tablets is described. The prednisolone was chromatographed using a ${\mu}-Bondapak\;C_{18}$ column and the eluent 70% MeOH at a flow rate 1. 0ml/min. Diethylstilbestrol was used as an internal standard. The UV detector response at 254nm was linear over a range of $10{\sim}60{\mu}g/ml$ under conditions of the analysis. Reproducibility studies gave relative standard deviations of $0.3{\sim}0.5%$.