한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제10권3호
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- Pages.197-203
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- 1982
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Verticillium sp. 가 생산하는 Protopectin 용해효소에 관한 연구 (제 2 보) 효소의 정제 및 성질
Studies on the Protopectinase Produced by Verticillium sp. (Part 2) Purification and Properties of Protopectinase from Verticillium sp.
초록
The protopectinase from the culture extract of a Verticillium sp. was purified about 1000 fold by ammonium sulfate fractionation, DEAE-Sephadex treatment and Sephadex G-75 column chromatography. The purified enzyme was homogeneous on electrophoresis and its molecular weight was estimated to be 38000 by Andrew's gel filtration, method. The enzyme was almost stable under the temperature of 4
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