Aspergillus niger KUF-04에 의한 Glucose Oxidase 생산조건에 관한 연구

Studies on the Conditions of Glucose Oxidase Production by Aspergillus niger KUF-04

토양에서 분리 동정된 Aspergillus niger KU K-04에 의한 glucose oxidase 생산을 최대화하기 위하여 효소생산에 미치는 배양조건 및 영양원에 대하여 연구하였던 바 다음과 같은 결과를 얻었다. 1. 효소생성을 위한 최적배양온도, 배지pH 및 배양시간은 각각 28~34$^{\circ}C$, 7.0~8.0 및 40 시간이었다. 2. 탄소원으로서는 포도당이 가장 양호하였으며 최적농도는 15% (w/v)이었다. 3. (NH$_4$)$_4$SO$_4$가 질색원으로서 가장 좋은 효소활성을 나타냈으며 효소생산을 위한 최적농도는 0.02%이었다. 4. 무기염으로서는 MgSO$_4$. 7$H_2O$ 및 KH$_2$PO$_4$를 각각 0.05%와 0.02%를 첨가했을 때 효소생산이 증가되었다.

To maximize the production of glucose oxidase by Aspergillus niger KUF-04 isolated from a soil, the cultivation conditions and nutrient sources for the enzyme production were studied. The results obtained were as fellows: 1. The optimum temperature, pH of the medium, and cultivation time for the enzyme formation were found to be 28-34$^{\circ}C$, 7.0-8.0 and 40 hours, respectively. 2. The best carbon source was proved to be glucose and its most effective concentration was 15 percent. 3. Ammonium sulfate was the best nitrogen source as compared with the other inorganic and organic nitrogen sources tested. Its optimum concentration for the glucose oxidase production was 0.02 percent. 4. As mineral sources, 0.05% of Mag cesium sulfate 7-hydrate and 0.02% of Potassium phosphate, monobasic seemed to be necessary to further increase the level of the enzyme production.