가토 적혈구 세포막 $Na^+,\;K^+-ATPase$활성에 미치는 Carbachol의 영향

The Effect of Carbachol on $Na^+,\;K^+-ATPase$ Activity in Rabbit Erythrocyte Membrane

  • 김옥진 (서울대학교 약학대학 약효학교실) ;
  • 김낙두 (서울대학교 약학대학 약효학교실) ;
  • 박찬웅 (서울대학교 의과대학 약리학교실) ;
  • 홍사악 (서울대학교 의과대학 약리학교실)
  • Kim, Ok-Jin (Department of Pharmacology, College of Pharmacy, Seoul National University) ;
  • Kim, Nak-Doo (Department of Pharmacology, College of Pharmacy, Seoul National University) ;
  • Park, Chan-Woong (Department of Pharmacology College of Medicine, Seoul National University) ;
  • Hong, Sa-Ack (Department of Pharmacology College of Medicine, Seoul National University)
  • 발행 : 1982.12.30

초록

$Na^+,\;K^+-ATPase$ is a component of plasma membrane in almost all animal cell, and maintains ionic distribution and membrane potential of normal cell. In the mechanism of adrenergic transmission, it is relatively well known that drug-receptor combination leads to stimulate adenylate cyclase and so on. In the cholinergic transmisison, the mechanism is not well known but is simply interpreted as the change of membrane permeability results from acetylcholine receptor interaction. To study the relationship between cholinergic transmission and membrane $Na^+,\;K^+-ATPase$, the effect of carbachol on $Na^+,\;K^+-ATPase$ activity in rabbit erythrocyte membrane is studied. The results are summarized as follows. 1) Total ATPase, $Mg^{+2}-ATPase$ and $Na^+,\;K^+-ATPase$ of rabbit erythrocyte membrane show maximum activities at 1mM of tris-ATP. 2) Total ATPase activity tends to increase when treated with carbachol $(10-^{-9}M-10^{-3}M)$. 3) The $Mg^{+2}-ATPase$ activity also tends to increase when treated with carbachol $(10-^{-9}M-10^{-3}M)$. 4) The $Na^+,\;K^+-ATPase$ activity is inhibited when treated with carbachol $(10-^{-9}M-10^{-7}M)$. It is suggested that the inhibition of $Na^+,\;K^+-ATPase$ by cholinergic drugs may be considered as one part of mechanism of cholinergic transmission.

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