한국대두에서의 Trypsin Inhibitor Variant의 분포와 유전

Distribution and Inheritance of Trypsin Inhibitor Variant in Soybeans Grown in Korea

한국에서 재배되고 있는 대두에서의 trypsin inhibitor variant의 분포를 조사하기 위하여 51 품종의 종실 단백질을 disc electrophoresis에 의하여 분리했다. 9품종이 Rf 0.79 band를, 그리고 42품종이 Rf 0.83 band를 갖고 있었으며, Rf 0.79의 분리비율은 17.6% 이었다. 금강대입(Rf 0.79 band)과 의두(Rf 0.83band)의 F_1은 Rf 0.79 band와 Rf 0.83 band를 모두 갖고 있었으며, F_2에서의 Rf 0.79:Rf 079/Rf 0.83:Rf 083의 분리는 22:53:21로서 이들 soybean trypsin inhibitor variant가 1쌍의 codominant alleles에 의하여 지배됨을 나타냈다.

Seed proteins of 51 varieties of soybean were separated by disc electrophoresis. Nine varieties had Rf 0.79 electrophoretic band, and 42 varieties had Rf 0.83 band. The frequency for Rf 0.79 was 0.176 and the frequency for Rf 0.83 was 0.824. The F_1 seeds of crossed between Kumgangdaepp (Rf 0.79) and Uidu (Rf 0.83) possessed both bands. Analysis of 96 F_2 seeds showed a ratio of 22 : 53 : 21 (Rf 0.79 : Rf 0.79/Rf 0.83 : Rf 0.83), suggesting single gene control with two co dominant alleles.