한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제9권4호
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- Pages.197-205
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- 1981
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
미생물 페니실린 아미다제에 관한 연구 (제 6 보) 흡착효소의 아크릴아마이드젤 포괄방법에 의한 Bacillus megaterium의 변이주가 생산하는 페니실린 아미다제의 고정화에 관한 연구
Studies on Microbial Penicillin Amidase (Part 6) Immobilization of Penicillin Amidase from Bacillus megaterium by Adsorption and Acrylamide Gel Entrappment
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Seong, Baik-Lin
(Biotechnology Research Department Korea Advanced Institute of Science and Technology) ;
- Son, Hyeung-Jin (Biotechnology Research Department Korea Advanced Institute of Science and Technology) ;
- Mheen, Tae-Ick (Biotechnology Research Department Korea Advanced Institute of Science and Technology) ;
- Moon H. Han (Biotechnology Research Department Korea Advanced Institute of Science and Technology)
- 발행 : 1981.12.01
초록
Bacillus megaterium의 발효액으로 부터 페니실린 아미다제를 셀라이트에 흡착시켜 분리한 후 이 흡착효소를 아크릴아마이드에 포괄시켜 고정화하였다. 관형식 반응조에서의 이 고정화효소의 안정도는 포괄시키지 않은 흡착효소에 비해 크게 증가하였으며 최적 반응 pH는 8.7, 그리고 최적 안정도는 7.5~8.0이었고 최적온도는 5
Penicillin amidase of Bacillus megaterium was recovered from the fermentation broth by adsorption on celite and immobilized by entrapping the adsorbed enzyme in acrylamide gel. The operational stability in column reactor was greatly increased by entrappment as compared with that of without entrappment. The optimum pH of the immobilized enzyme was 8.7 with broader activity profile than that of the free enzyme, while the most stable pH range appeared to be between pH 7.5 and 8.0. The optimum temperature was shifted to 5
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