미생물(微生物) 고정화(圖定化)에 관한 연구(硏究) - 제1보(第1報). Lactobacillus 균(菌)의 고정화조건(圖定化條件) -

Immobilization of Microorganisms - Part 1. Preparation of Immobilized Lactobacillus bulgaricus -

Lactobacillus bulgaricus 균체(菌體)의 고정화방법(圖定化方法)으로서 polyacrylamide gel과 Al- Ca-, Fe- 및 Mg-alginate bead에 대하여 고찰하고 이중 가장 활성이 좋은 것은 Ca-alginate bead 이었으며 입경(粒經) 2m/m로 형성시킨 Ca-alginate bead로 고정화(圖定化)시킨 균류(菌類)의 경우, 4.5% lactose 용액 및 whey에서의 lactose 분해실험 결과, 생균(生菌)에 대비(對比)한 상대적 활성은 각각 최고 28% 및 18% 이었다.

The immobilization of Lactobacillus bulgaricus was investigated by various method, e.g. by use of polyacrylamide gel and Al-, Ca-, Fe- or Mg-alginate beads, and the most active immobilized cells were obtained by entrapment in a Ca-alginate beads. These immobilized microbial cells, when introduced into 4.5% lactose solution and whey solution showed maximum relative activity of 28% or lactose solution and 18% for whey solution as measured against the native microbial reference standard (100).