Applied Biological Chemistry
- 제24권2호
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- Pages.85-93
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- 1981
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
섬유소분해(纖維素分解)의 미생물학적(微生物學的) 연구(硏究) - 제1보(第一報). 섬유소분해(纖維素分解) 미생물(微生物)의 분리(分離) 및 효소특성연구(醉素特性硏究) -
Studies on the Microbial Decomposition of Cellulosic Materials - Part Ⅰ. Isolation of Cellulase-producing Microorganisms and Characterization of the Enzyme Activities -
- Kim, Kyo-Chang (Department of Agricultural Chemistry, Chung-Buk National University) ;
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Kim, Chi-Kyung
(Department of Biology, Chung-Buk National University) ;
- Kim, Chang-Han (Department of Science Education, Chung-Buk National University)
- 발행 : 1981.06.30
초록
섬유소(纖維素)를 이용(利用)하기 위한 기초연구(基礎硏究)로서 섬유소분해능(纖維素分解能)이 우수(優秀)한 균주(菌株)를 분리(分離)하여 동정(同定)한 결과(結果) Trichoderma spp.로 추정하였으며 사용된 섬유소 기질(基質)은 볏짚을 용제(溶劑)로 전처리(前處理)한 것과 여기에 산, 가열처리(加熱處理)를 더 가(加)한 것을 사용(使用)하였으며 이 미생물(微生物)에 의(依)하여 전처리 및 산 가열처리(加熱處理)에서 생성되는 섬유소 분해 효소(酵素)를 시험한 결과(結果) 0.1%
For the utilization of natural cellulosic materials by microorganisms, a potent cellulase-producing microorganism was isolated and identified as Trichoderma spp. Rice straw used as a substrate in this study was preliminarily treated with chemical solvents and/or additionally treated with acids and by heat, and then examined with the cellulase produced by the organism. Better results in sugar production by decomposing the straw cellulose were obtained, when the cellulase was produced by cultivating the organism in the selection medium, pH 5.0, for 5 days, and when the pretreated straw substrate was additionally treated with 0.1%
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