대한미생물학회지 (The Journal of the Korean Society for Microbiology)
- 제15권1호
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- Pages.71-75
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- 1980
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- 0253-3162(pISSN)
T subset정량(定量)을 위한 항우적혈구(抗牛赤血球) IgG항체(抗體)의 분리.정제(分離.精製)(I)
Purification of Anti-ox Red Blood Cell IgG Antibody for T subset Assay
- Ha, Youn-Mun (Department of Microbiology, Kyung Hee University, College of Medicine) ;
- Lee, Jean-Yong (Department of Microbiology, Kyung Hee University, College of Medicine) ;
- Lim, Soo-Duk (Department of Dermatology, Kyung Hee University, College of Medicine)
- 발행 : 1980.12.31
초록
사람에 있어서 면역(免疫)담당세포의 하나인 T세포(細胞)는 몇몇 subpoulation으로 나누어지고 있으며 그중
Antisera to ox red blood cell were prepared by intraperitoneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for
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