The Journal of the Korean Society for Microbiology (대한미생물학회지)
- Volume 15 Issue 1
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- Pages.71-75
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- 1980
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- 0253-3162(pISSN)
Purification of Anti-ox Red Blood Cell IgG Antibody for T subset Assay
T subset정량(定量)을 위한 항우적혈구(抗牛赤血球) IgG항체(抗體)의 분리.정제(分離.精製)(I)
- Ha, Youn-Mun (Department of Microbiology, Kyung Hee University, College of Medicine) ;
- Lee, Jean-Yong (Department of Microbiology, Kyung Hee University, College of Medicine) ;
- Lim, Soo-Duk (Department of Dermatology, Kyung Hee University, College of Medicine)
- Published : 1980.12.31
Abstract
Antisera to ox red blood cell were prepared by intraperitoneal hypermultiple injections without adjuvant in outbred white rabits. Purified IgG fraction from these rabbits anti-ox red blood cell antiserum for T subset assay was obtained by precipitation with 50% saturated ammonium sulphate followed by DEAE-cellulose chromatography and Sephadex G-150 gel filtration. These purified IgG fraction was compared with Cappel company standard IgG fraction for
사람에 있어서 면역(免疫)담당세포의 하나인 T세포(細胞)는 몇몇 subpoulation으로 나누어지고 있으며 그중
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