The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
권호 표지

A Study on the Quality Control of 2-site Immunoradiometric Assay of Serum Ferritin

혈청(血淸) ferritin 측정(測定)의 정도관리(精度管理)에 관(關)한 고찰(考察)

  • Kim, Byoung-Kook (Dept. of Internal Medicine, Seoul National University Hospital) ;
  • Seo, Il-Tack (Dept. of Nuclear Medicine, Seoul National University Hospital) ;
  • Kim, Kwang-Won (Dept. of Internal Medicine, Seoul National University Hospital) ;
  • Cho, Bo-Yeon (Dept. of Internal Medicine, Seoul National University Hospital) ;
  • Koh, Chang-Soon (Dept. of Internal Medicine, Seoul National University Hospital) ;
  • Lee, Mun-Ho (Dept. of Internal Medicine, Seoul National University Hospital)
  • 김병국 (서울대학교 의과대학 내과학교실) ;
  • 서일택 (서울대학교병원 핵의학부) ;
  • 김광원 (서울대학교 의과대학 내과학교실) ;
  • 조보연 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 이문호 (서울대학교 의과대학 내과학교실)
  • Published : 1980.06.30
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Abstract

A 2-site immunoradiometric assay for serum ferritin was evaluated with commercially available kit. The assay required 6 hours. The slope of the standard curve kept up ideal range with the calculation of maxium binding instead of total dose until expire date. The stage II washing was more important than the stage I washing on the modified washing procedure as the bead keeping to remain in the tube. With this modified mothod, three times of tube. washing was sufficient to reduce the significant errors The measured values of serially diluted sample with standard diluting buffer was proportional to the predicted values. In the experiment of serum effect on the assay. a linear relationship from 5 to 50% serum, but beyond 50% there was reduction in measured ferritin concentration. It has a sensitivity of 2.77 ng/ml, within-assay precision (CV) of 8.0%, and between-assay reproducibility(CV) of 7.4% (mean 174.8 ng/ml).

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