한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제8권2호
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- Pages.125-133
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- 1980
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
포도당 이성화효소 생산균의 동정 및 그 효소의 부분정제
Identification of a Bacterium which Produced D-Glucose Isomerase and Partial Purification on the Enzyme
- Rhee, In-Koo (Department of Agricultural Chemistry, College of Agriculture, Kyungbook National University) ;
- Seu, Jung-Hwn (Department of Agricultural Chemistry, College of Agriculture, Kyungbook National University)
- 발행 : 1980.06.01
초록
토양으로부터 분리한 포도당 이성화효소를 강하게 생산하는 방사균을 Bergey's manual 8판에 따라 동정한 결과 Streptomyces antibioticus 근록의 균주이었다. 본 균의 배양액으로부터 균체를 모아서 해사를 넣고 파쇄 하여 증류수로 추출하고 Mn-처리를 하여 핵단백질을 제거한 후 황산 ammonia 분획침전(0.5∼0.8포화), 수석, DEAE-cellulose column chromatography, DEAE-sephadex (A-50) column chromatography 및 sephadex G-200에 의한 gel filtration을 거쳐 비활성도로 약 380배, 회수율 25％ 정도로 분리 정제하였다.
A microorganism which produced D-glucose isomerase was identified to be similar to Streptomyces antibioticus on the morphological, cultural and physiological characteristics except the spore chain and the utilization of sucrose. D-xylose grown cells of Streptomyces sp. strain K-17 were disrupted by grinding with sea sand. D-glucose isomerase was partially purified with the fractionation by ammonium sulfate, Mn-treatment, DEAE-cellulose column chromatography, DEAE-sephadex (A-50) column chromatography and gel filtration of sephadex G-200. The enzyme was purified about 380 fold with 25 ％ recovery.
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