미생물학회지 (Korean Journal of Microbiology)
- 제18권3호
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- Pages.133-147
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- 1980
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Aspergillus nidulans FGSC 159의 carboxymethylcellulases의 분리 순화 및 그 성질에 관한 연구
Purification and Properties of Carboxymethylcellulases from Aspergillus nidulans FGSC 159
초록
Washed mycelia of Aspergillus nidulans FGSC159 were incubated in CMC minimal liquid medium and the culture filtrate which contained induced extracellular cellulase was fractionated by a three-step procedure including chromatography on Bio-Gel P-150, chromatography on DEAE-Sephadex A-50 and chromatography on Sephadex G-100. Three CMCase components ; F-I-Ia, F-I-Ib and F-II-Ia were prepared. No enzyme activity toward avicel could be detected in these components. Similarly, there was no
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