Effects of Inoculation of Nodule Bacteria (Rhizobium meliloti) on the Nodule Formation and Plant Growth of Alfalfa (Medicago sativa L.)

근류균(根瘤菌)(Rhizobium meliloti)의 접종(接種)이 Alfalfa의 근류형성(根瘤形成) 및 초기생육(初期生育)에 미치는 영향(影響)

  • Kim, Choong Soo (Dept. of Agronomy, Coll. of Agriculture, Chungnam Natl. Univ.)
  • 김충수 (충남대학교 농과대학 농학과)
  • Published : 1979.12.31

Abstract

This experiment was carried out to define the affinity of alfalfa varieties to nodule bacteria and the effects of nodule bacteria on the growth of alfalfa varieties. The results obtained are summarized as follows; 1. The ability of nodule formation on the roots of alfalfa was significantly different among the strains of nodule bacteria, Rhizobium meliloti SU 47 formed nodules on the roots of two alfalfa varieties, Luna and Von arnims altd bost. Nodules were not formed at the roots of all varieties by inoculation of Rhizobium meliloti M1 but a lot of nodules were formed at the roots of all varieties by inoculation of Rhizobium meliloti M14. 2. When the Rhizobium meliloti M14 was inoculated in the media, the largest numbers of nodules were formed on the roots of alfalfa cultivar Franks hangmeiler and the least numbers of nodules were formed on the roots of cultivar Elga. 3. The plant height and the dry matter weight of all alfalfa cultivars were significantly increased by inoculation of Rhizobium meliloti M14, and also those were increased by applying nitrogen, but when the alfalfa cultivars were grown on the media inoculated Rhizobium M1, the plant height and the dry matter weight of alfalfas were not increased and they were same as those grown on check media. 4. The significant correlation were not detected between the number of nodules formed on the, roots of alfalfa cultivars and the plant height or the dry matter weight of alfalfa cultivars.

몇가지 Alfalfa 품종(品種)의 근류균(根瘤菌)에 대(對)한 친화성(親和性) 및 접종효과(接種效果)를 구명(究明)코자 시험(試驗)하였던 바 결과(結果)를 요약(要約)하면 다음과 같다. 1. 접종균주간(接種菌株間)에는 근류형성성(根瘤形成性)에 현저(顯著)한 차이(差異)를 보였으며 Rhizobium meliloti SU47균주(菌株)는 Luna, Von arnims altd bost 등(等) 두 품종(品種)에만 근류(根瘤)를 형성(形成)하였으며 M1균주(菌株)는 전품종(全品種)에서 근류(根瘤)를 형성(形成)치 않았고 M14균주(菌株)는 전품종(全品種)에서 근류(根瘤)를 형성(形成)하였다. 2. 근류착생수(根瘤着生數)는 Franks hangmeiler 품종(品種)이 가장 많았고 그 다음이 Galilee 품종(品種)이었으며 Elga 품종(品種)이 가장 적었다. 3. 초장(草長) 및 지상부건물중(地上部乾物重)은 공시품종(供試品種) 모두 M14균주접종구(菌株接種區)가 가장 컸고 그 다음이 질소대조구(窒素對照區)였으며 M1접종구(接種區)와 대조구(對照區)에서 가장 작았고 근장(根長) 및 지하부건물중(地下部乾物重)은 품종간(種間)및 처리간(處理間)에 유의성(有意性)을 인정(認定)할 수 없었다. 4. 근류착생수(根瘤着生數)와 초장(草長) 및 근장(根長) 그리고 지상부건물중(地上部乾物重)과 근건물중(根乾物重)과는 상관관계(相關關係)를 인정(認定)할 수 없었다.

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