Subunit Interactions of Vertebrate Lactate Dehydrogenase: I. Immunochemistry of Subunits

Two homotetrameric lactate dehydrogenase isozymes from Fluta alba and one from Ophicephalus argus were purified by combination of gel filtration and DEAE-cellulose chromatogrphy. The final preparations were isozymically pure and used to elicit antibodies in rabbits. The immunochemical reactivities demonstrated that the amino acids of active site is not to be included in the antigenic determinants, that antibodies or unknown component of immunized rabbit serum might be responsible for the electrophoretic abnormality and that two subunits share common antigenic determinants, reflecting that these polypeptides have a common evolutionary origin.

경골어류 드렁허리와 가물치에서 젖산수소이탈효소의 세가지 동질사량체를 순수 정제하였다. 이들에 대한 항혈청을 얻어 젖산수소이탈효소와 면역화학적 반응을 실시하였다. 하부단위체의 활성자리는 항원결정군에 속해 있지 않으며, 항혈청내의 항체 또는 미지의 물질이 하부단위체의 하전을 변화시키며, 두 하부단위체는 공통의 항원결정군을 갖고 있음이 확인되었다.