한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제11권2호
- /
- Pages.77-80
- /
- 1979
- /
- 0367-6293(pISSN)
녹차중(綠茶中)의 L-Ascorbic Acid의 정량법(定量法)에 관한 연구(硏究)
Analytical Method of L-Ascorbic Acid Content in Green Tea
- Shin, Mee-Gyung (Won Kwang University) ;
- Nam, Chang-Woo (Dong Duk Women's College)
- 발행 : 1979.06.30
초록
녹차중(綠茶中)의 L-ascorbic acid를 정량(定量)함에 있어서 흔히 사용되고 있는 DNP법(法)을 적용할 때 불순물(不純物)에 의한 방해(妨害)여부와 그의 제거방법(除去方法)에 대하여 검토(檢討)한 결과(結果)는 다음과 같다. 즉, DNP법(法)으로 AsA를 측정시 ethyl glyoxal, diacetyl, caffeine둥 불순물질 및 아미노산(酸)들이 방해하였으며, 이들 물질을 제거하는 수단으로 브롬산화(酸化)후 o-phenylenediamine을 반응시킨 다음 chloroform으로 추출제거(抽出除去)하고 Amberlite
Effects of interfering substances on the determination of L-ascorbic acid (AsA) in green tea and it's extracts by 2,4-dinitrophenylhydrazine (DNP) method was studied. and the removal of these interfering substances was also investigated. Under the condition prescribed for DNP method, AsA content of green tea are effected by some sugar, reductones, dicarbonyl compounds, organic acids, amino acids and others. All interfering substances except amino acids were eliminated by the chloroform extraction after adding o-phenylendiamine to sample solution. and remaining amino acids were eliminated almost completely by the treatment with ion exchange resin
키워드