대한미생물학회지 (The Journal of the Korean Society for Microbiology)

대한미생물학회 (The Korea Society for Microbiology)
권호 표지

비색소성(非色素性) Serratia marcescens의 분리(分離) 동정(同定)

Identification of non-pigmented Serratia marcescens

  • 안무식 (경북대학교 의과대학 세균학교실) ;
  • 정재규 (경북대학교 의과대학 세균학교실) ;
  • 조동택 (경북대학교 의과대학 세균학교실)
  • Ahn, Moo-Sik (Department of Bacteriology, Kyungpook National University, School of medicine) ;
  • Chung, Jae-Kyu (Department of Bacteriology, Kyungpook National University, School of medicine) ;
  • Cho, Dong-Taek (Department of Bacteriology, Kyungpook National University, School of medicine)
  • 발행 : 1978.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Serratia marcescens의 동정(同定)을 분리배지(分離培地)를 이용(利用)하여 간편(簡便)한 방법(方法)으로 시도하였던 바 prodigiosin 산생(産生)은 proline을 위시해서 각종(各種) amino acid, thiamine 및 nicotinic acid에 의해서 항진되지 않았으나 혈액평판배지(血液平板培地)에서 본(本) 균종(菌腫)은 시간(時間)의 경과(經過)에 따라 흑색조(黑色調)를 띤 집락(集落)을 형성(形成)하며 이는 일차적(一次的)인 분리기준(分離基準)으로 이용(利用)될 수 있을 것 같다. Tween 80 및 대두유(大豆油) 분해성(分解性은) 본(本) 균(菌)에서 양성(陽性)이며 동정(同定)에 이용(利用)될 수 있으나 특이반응(特異反應)이 아니다. 그러므로 마지막으로 oxidase 반응(反應), lactose 및 sucrose 발효시험이 본(本) 균종(菌腫)의 확정(確定)에 수반되어야 한다.

Among clincal isolates, most strains of Serratia marcescens were belonged to nonpigmented form, and several attempts were undertaken for the rapid and simple identification of these strains. Prodigiosin production of non-pigmented strains was uniformly negative in many kinds of solid media as well as in nutrient agar added with various amino acids and thiamine. On blood agar, colonies of S. marcescens turned gradually to grey or dark color by the lapse of incubation period and this characteristic seems to be able to utilize as an indicator for a primary isolation, and also generally paralleled with the results of dehydration of Tween 80 and lipase activity in soy bean oil medium although these reactions were by no means specific to S. marcescens. In order to rule out these non-specific reactions, other tests such as oxidase and sucrose fermentation are required for the final confirmation of this species.

키워드