응유효소의 간편한 역가측정법

A Simple Method for Determining Activity of Milk Clotting Enzymes

  • Shin, H.K. (Department of Biological Science and Engineering Korea Advanced Institute of Science) ;
  • Pack, M.Y. (Department of Biological Science and Engineering Korea Advanced Institute of Science)
  • 발행 : 1978.09.01

초록

효소에 의한 우유의 응고과정은 효소적인 단계와 비효소적인 단계의 두 가지로 구성되어 있다는 사실이 확인되었다. 효소적인 단계가 끝나는 점을 시험관속에서 반응을 시킨 다음 그 시험관을 5도의 경사까지 기울었을 때 우유가 시험관 벽을 타고 흐를수 있는 거리를 가지고 측정하였다. 효소적인 단계에 소요되는 반응시간을 측정하므로서 응유효소의 역가를 평가하는 새로운 방법이 제시되었다.

The process of milk clotting by enzymes was confirmed to be consisted of two distinct stages: an enzymic coagulation stage followed by a nonenzymic clotting stage. The endpoint of the enzymic stage was determined simply by measuring flow distance of milk-enzyme system in a regular test tube being laid down to five degree slope after the reaction. By measuring the time elapsed during the enzymic stage a new method of evaluating the power of milk clotting enzymes was proposed.

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