Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 6 Issue 3
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- Pages.109-113
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- 1978
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
A Simple Method for Determining Activity of Milk Clotting Enzymes
응유효소의 간편한 역가측정법
- Shin, H.K. (Department of Biological Science and Engineering Korea Advanced Institute of Science) ;
- Pack, M.Y. (Department of Biological Science and Engineering Korea Advanced Institute of Science)
- Published : 1978.09.01
Abstract
The process of milk clotting by enzymes was confirmed to be consisted of two distinct stages: an enzymic coagulation stage followed by a nonenzymic clotting stage. The endpoint of the enzymic stage was determined simply by measuring flow distance of milk-enzyme system in a regular test tube being laid down to five degree slope after the reaction. By measuring the time elapsed during the enzymic stage a new method of evaluating the power of milk clotting enzymes was proposed.
효소에 의한 우유의 응고과정은 효소적인 단계와 비효소적인 단계의 두 가지로 구성되어 있다는 사실이 확인되었다. 효소적인 단계가 끝나는 점을 시험관속에서 반응을 시킨 다음 그 시험관을 5도의 경사까지 기울었을 때 우유가 시험관 벽을 타고 흐를수 있는 거리를 가지고 측정하였다. 효소적인 단계에 소요되는 반응시간을 측정하므로서 응유효소의 역가를 평가하는 새로운 방법이 제시되었다.
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