Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 6 Issue 1
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- Pages.1-8
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- 1978
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Studies on the Microbial Utilization of Agricultural Wastes (Part 11) Properties of Cellulolytic Enzyme Produced by a Cellulolytic Fungus Trichodrma sp. KI 7-2 and its Application to the Fermented Feed Production
농산폐자원의 미생물학적 이용에 관한 연구(제11보) Trichoderma sp KI 7-2가 생산하는 섬유소분해효소의 성질 및 발효사요에의 응용
- Bae, Moo (Applied Microbiology Lab., Korea Institute of Science & Technology) ;
- Lee, Gye-Jun (Applied Microbiology Lab., Korea Institute of Science & Technology) ;
- Tak, Sun-Mi (Applied Microbiology Lab., Korea Institute of Science & Technology) ;
- Kim, Byung-Hong (Applied Microbiology Lab., Korea Institute of Science & Technology)
- 배무 (한국과학기술연구소 응용미생물연구실) ;
- 이계준 (한국과학기술연구소 응용미생물연구실) ;
- 탁선미 (한국과학기술연구소 응용미생물연구실) ;
- 김병홍 (한국과학기술연구소 응용미생물연구실)
- Published : 1978.03.01
Abstract
In order to develop the processes for the production of fermented feed from cellulosic agricultural by-product, cereal straw, by th action of cellulolytic fungus, the properties of the cellulolytic enzyme produced by Trichoderma sp. KI 7-2 was studied. A higher enzyme activity was obtained in the culture added by 1% rice or barley straw powder than in the culture of pure cellulose. The crude enzyme was prepared by precipitating from 20∼60% saturated ammonium sulphate of the culture supernatant. The optimum conditions for the enzyme reaction were temperature of of 50
섬유소분해효소 생산균인 Trichoderma sp. KI 7-2의 cellulase생성을 위한 탄소원으로는 순수한 섬유소보다 볏짚 보리짚 분말을 사용하는 것이 좋았으며 1% 첨가에서 심부배양한 액을 유안농도 20~60%로 떨어뜨린 침전에서만 cellulase의 활성이 나타났다. 이 조효소의 작용최적온도는 5
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