한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제10권2호
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- Pages.181-188
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- 1978
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- 0367-6293(pISSN)
조직배양(組織培養)에 의한 인삼성분(人蔘成分)의 변화(變化) -제1보(第一報) Callus와 인삼성분(人蔘成分)의 비교(比較)-
Study on the Changes in Saponins from Ginseng Callus by Tissue Culture -Part 1. Comparison of Saponins from Callus Tissue and from the Root of Ginseng Plant-
- Yang, R. (The Graduate School of Engineering, Yonsei University) ;
- Choi, Y.C. (The Graduate School of Engineering, Yonsei University) ;
- Kim, H.J. (Laboratory of IL HWA CO., LTD.) ;
- Lee, S.C. (Laboratory of IL HWA CO., LTD.) ;
- Park, S.H. (Laboratory of IL HWA CO., LTD.)
- 발행 : 1978.04.30
초록
1년생(年生) 수삼근(水蔘根)과 엽병(葉柄)(petiole)으로 조직배양(組織培養)한 callus 양자간(兩者間)의 유효성분(有效成分)인 saponins 및 sapogenins에 관하여 비교(比較) 검토(檢討)한 결과(結果)는 다음과 같다. (1) 인삼(人蔘)과 callus는 Liebermann-Burchard 반응(反應)에서 양성(陽性)으로 확인(確認)되었다. (2) Ginsenoside
To study on the changes in saponins from callus mass by tissue culture, the callus was derived from the petiole of Korean Ginseng (Panax Ginseng C.A. Meyer) and cultivated on Murashige and Skoog's agar medium supplemented with 2.4-dichlorophenoxyacetic acid and kinetin for 8 months. Then, well-grown callus was analyzed for its components estimation. The results obtained are as follows: (1) When saponins isolated from callus mass were chromatographed on a silca gel plate, and determined by the thinchrograph TFG-10, the ratio of Rb, c to Rg(f) in saponins was 2.16 to 1 and Rb, c, d to Re, g (f) was 1 to 1.63, while in the case of saponins from the root of Panax Ginseng grown by soil culture, the ratio of Rb, c to Rg(f) was 1.03 to 1 and the ratio of Rb, c,d to Re, g(f) was 1 to 1.17. (2) Sapogenins were obtained from the hydrolysates of saponins, and determined by thinchrograph TFG-10. The ratio of panaxadiol to panaxatriol in sapogenins from callus saponins was 2.66 to 1, while the ratio of panaxadiol to panaxatriol in sapogenins from ginseng root saponins was 1.86 to 1. From the results above mentioned, we concluded that the relative contents of sapogenins in saponins from callus mass by tissue culture were different from those in saponins from ginseng root by soil culture.
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