열대산(熱帶産) 광엽수재(廣葉樹材)의 Xylan에 관(關)한 연구(硏究) (II) -Xylan의 단리(單離) 및 정제(精製)-

Studies on Xylan of Tropical Hardwood (II) -Isolation and Purification of Xylan-

아염소산염법(亞鹽素酸鹽法)에 의하여 대부분(大部分) 탈(脫) lignin된 holocellulose를 얻었다. Alkali농도별(濃度別)의 추출(抽出)에서는 5% KOH로 3회추출(回抽出)하여도 일부(一部)의 xylan이 잔존(殘存)하며 타(他)의 hemicellulose와 cellulose의 분해성물질(分解生成物)이 얻어지며, 10%와 24% KOH추출(抽出)은 그와 같이 좋은 결과(結果)가 얻어졌다. Xylan의 침전단리(沈澱單離)에 다량(多量)의 ethanol을 사용(使用)하는 상법(常法)보다는 약(約) 1/10로 농축(濃縮)하여 cellophane막(膜)에 의한 투석법(透析法)으로 소량(少量)의 ethanol이라도 회수율(回收率)과 높은 순도(純度)의 xylan이 얻어졌다. Glucornannan의 단리(單離)에 있어서 5% KOH 추출잔사(抽出殘査)는 xyJan함유량(含有量)이 많고 10%와 24% KOH 추출잔사(抽出殘査)는 그의 같은 결과(結果)이며, glucose와 mannose의 비(比)는 약(約) 1 : 1이다. Fehling용액정제(溶液精製)는 비교적(比較的) 순수(純粹)한 xylan을 얻지만, 분화분해(孵化分解), 조작(操作)의 번잡(煩雜) 및 회수율(回收率)이 낫고, 그다지 좋은 방법(方法)은 아니다. Ethanol적하법(滴下法)은 1일(日)정제(精製)라도 고수율(高收率) 및 고순도(高純度)의 xylan이 얻어지고 조작(操作)의 영역(容易)등을 고려(考慮)하여 대단히 우수한 xylan 정제법(精製法)이다. 그러나 양정제법(兩精製法)으로 arabinose잔기(殘基)를 완전(完全)히 제거(除去)할 수는 없었다. 이것은 후보(後報)에 검토(檢討)를 보고(報告)한다.

According to the chlorous salt method, most of holocellulose whose lignin was removed, was obtained. In extracting xylan from holocellulose by the different densities of alkali, 5% KOH was extracted three times but still there remained part of xylan in it and another composite of hemicellulose and cellulose was obtained. The extraction of 10% and 20% KOH showed a desirable result. Rather than the ordinary method to use a large quantity of ethanol in the precipitation isolation of xylan, the method to use a small quantity of ethanol in adopting the dialysis with cellophane-membrane by condensing density to one tenth, made il possible to extract a high purity xylan in a high retrieving rate. In isolating glucomannan, the residue of 5% KOH extraction contained a large quantity of xylan, the residue of 10% and 24% KOH extraction, also showed the same result and the comparison between glucose and mannose was approximately 1 : 1. The purification of Fehling solution made it possible to obtain comparatively pure xylan but the process of oxidation dissolution was complicated and the retrieving rate was low. This was not a good method. The ethanol titration purified a high purity xylan in a high retrieving rate and was a very excellent purifying method, considering its simple and easy process. These two purifying methods, however, could not completely remove the residue of arabinose. This will be examined and reported later.