Optimal conditions for pigmentation in Bacillus licheniformis SSA3 and cloning of a DNA fragment involved in pigment production

  • Kim, Jong-Kyu (Department of Applied Microbiology, Yeungnam University) ;
  • Shin, Ok-Sun (Department of Applied Microbiology, Yeungnam University)
  • 발행 : 1995.03.01

초록

Bacillus licheniformis SSA3 can produce a dark-brown antimutagenic pigment. The optimal conditions for production of this pigment are reached at 0.1% tyrosine, in pH 6-8, within 7-9 days, at $30^{\circ}C$, and in aerobic condition. We cloned a DNA fragment involved in pigment synthesis from Bacillus licheniformis SSA3 using a mutant strain. The cloned DNA was 7kb in size, which can produce the same pigment even in E. coli.

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