페니실린 아미다제 생산의 최적 조건 및 간이화한 효소 정량 방법에 대한 연구

Optimization of Penicillin Amidase Production and A Simplified Enzyme Assay Method

Penicillin amidase (EC 3.5.1.11) was produced by a mutant strain of Bacillius megaterium ATCC 14945. Hydroxylamine assay method for the determination of 6-APT was modified by using "HCl addition techniques" in order to simplify the time consuming orginal assay method without sacrifice of accuracy. Using the new mutant strain, the effects of fermentation conditions on enzyme production were studied.e studied.