Purification and Utilization of Industrial Waste Water Using Microorganism -(Part 1) Isolation of the yeast strain from organic waste water and its use on waste water treatment-

산업폐수의 처리 및 이용에 관한 연구 -(제 1 보) 효모균주의 분리와 이에 의한 유기성폐수의 처리에 관하여-

The yeast strain was isolated from food industry waste water and its identification and biological characteristics were investigated. The optimum condition for cultivations and its activities for the reduction of B.O.D. on the food industry waste water were also confirmed. The results are as follows; 1) The isolated was identified as Candida curvata. 2) Candida curvata grew well in all of the experimented media, so and it can be regarded as a useful strain in the treatment of food industry waste water. 3) There was only a slight difference in the induction period between sterilized cultivation and unsterilized cultivation. But in the ice cream waste water, the period was considerably longer in unsterilized cultivation. 4) Specific rate of growth of Candida curvata in sugar waste water was 0.50/hr, ice cream waste water 0.50/hr, and beer waste water 1.0/hr. 5) Increasing of innoculum reduced the induction period in unsterilized cultivation. 6) The amount of dried yeast from sugar waste water were $175mg/{\ell}$, ice cream waste water $628mg/{\ell}$, and beer waste water $857mg/{\ell}$. Crude protein content in the dried yeast from sugar waste water were 52%, ice cream waste water 54%, and beer waste water 54%. 7) The rate of BOD reduction in sugar waste water were 49%, ice cream waste water 80%, and beer waste water 64%.

산업폐수가운데 유기성폐수로서 설탕, Ice cream 및 맥주공장폐수등 3종을 택하여 그중에 번식하는 유용한 미생물로서 생장도활성이 큰 효모균주를 분리하고 선정균주에 의한 폐수처리실험한 결과 최적조건하($pH\;4.5{\sim}5.5,\;30{\pm}1^{\circ}C,\;O_2\;0.2\;v/v/min.,\;600{\sim}900\;r.p.m.$) 다음과 같은 결과를 얻었다. 1) 분리동정한 Candida curvata는 3종의 식품공장폐수에 배양실험한 결과, 무기영양원 첨가시 생육이 양호하여 폐수처리에 이용가능한 균주이었다 2) 폐수의 멸균조작의 유무는 설탕및 맥주공장폐수의 경우, 배양결과에 큰 차이가 없었으며, 다만 Ice cream공장폐수의 경우 비멸균시 induction period가 훨신 장시간이었다. 3) 비멸균배양시 최대 비증식속도는 설탕, Ice cream 및 맥주공장폐수 순으로 각각 0.5, 0.5 및 1.0/hr이었다. 4) 접종량을 늘리면, 비멸균시에서도 induction period가 짧아지고 따라서 배양시간이 단축되었다. 5) 건조균체량은 설탕, 맥주 및 Ice cream공장폐수 순으로 폐수 $1{\ell}$당 각각 175mg, 628mg 및 857mg이었고, 균체의 조란백함량은 같은 순으로 각각 52%, 54% 및 54%이었다. 6) BOD 제거율은 위의 5)항과 같은 순으로 각각 49%, 80%, 및 64%이었다.