Applied Biological Chemistry

  • 제20권1호
  • /
  • Pages.136-141
  • /
  • 1977
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

Porcine Leukocyte Lysosomal Hydrolase의 정제에 관(關)한 연구(硏究)

Purification of Porcine Leukocyte Lysosomal Hydrolases

  • Cho, Moo-Je (Department of Agricultural Chemistry, Gyeongsang National University)
  • 발행 : 1977.03.31
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초록

돼지 백혈구(白血球) Iysosome의 n-butanol 추출물(抽出物)로 부터 DEAE-cellulose, Sephadex c-200 column chromatography 및 Polyacrylamide gel electrophoresis 로서 acid phosphatase, aryl sulfatase, ${\beta}-glucuronidase$로서 및 cathepsin D를 분리(分離)정제하고 그 성질을 조사하였다.

DEAE-Cellulose, Sephadex column chromatography and polyacrylamide gel electrophoresis were used to purify acid phosphatase, aryl sulfatases, ${\beta}-glucuronidase$ and cathepsin D in n-butyl alcohol extracts of porcine leukocyte Iysosomes. The degree of purification was quite high for all enzymes studied and some could be identified by histochemical reactions.

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