한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제9권3호
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- Pages.183-189
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- 1977
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- 0367-6293(pISSN)
고정화 균체에 의한 Pyridoxl Phosphate의 생산에 관한 연구
Studies on the Formation of Pyridoxal Phosphate by Immobilized Cells
- 주영하 (경도대학농학부 농예화학과 발효생리학연구실) ;
- 곡길수 (경도대학농학부 농예화학과 발효생리학연구실) ;
- 이택수 (샘표 식품(주)연구실) ;
- 유태종 (고려대학교 농과대학 식품공학과)
- Chu, Young-Ha (Lab of Agricultural Chemistry, Kyoto University) ;
- Tani, Yoshiki (Lab of Agricultural Chemistry, Kyoto University) ;
- Lee, Taik-Soo (Lab. of Sampyo Foods Ind. Co., Ltd.) ;
- Yu, Tai-Jong (Department of Food Technology, College of Agriculture, Korea University)
- 발행 : 1977.09.28
초록
고정화(固定化) 균체(菌體)를 이용(利用)한 pyridoxal 5'-phosphate(pyridoxal-p)의 연속생산(連續生産)에 관(關)해실험(實驗)하였다. pyridoxine 5'-phosphate (pyridoxine-p) oxidase활성(活性)을 갖는 Pseudomonas polycolor 균체(菌體)와 Catalase 활성(活性)을 갖는 Kloeckera sp. No. 2201균체(菌體)를 효소원(酵素源)으로 사용(使用)하였다. 균체(菌體)는 Polyacrylamide gel에 불용화(不溶化) 시켰으며 동시고정균체(同時固定菌體)의 활성(活性)이 Pseudomonas Polycoler단독고정균체(單獨固定菌體)의 활성(活性)보다 강(强)하였다. 이 결과(結果)는 동시고정균체(同時固定菌體)의 Pyridoxine-p oxidase-cat-alase system은 Pyridoxine-p 산화(酸化)의 부산물(副産物)인
Studies were made of the continuous production of Pyridoxal 5'-phosphate (pyridoxal-p) on simultaneously immobilized cell column. Whole-cell of Pseudomonas polycolor having high activity of pyridoxine 5'-phosphate (pyridoxine-p) oxidase and Kloeckera sp. No. 2201 having high activity of catalase were used as the enzyme materials. The enzyme sources were entrapped in a polyacrylamide gel. Enzymatic properties of the simultaneously immobilized cells were investigated, comparing with those of the mixed whole-cells of the microorganisms. The simultaneously immobilized cells had higher enzyme activity than singly immobilized cells of Pseudomonas polycolor. From this result, the simultaneously immobilized pyridoxine-p oxidase-catalase system could be available to exert a protective effect upon the pyridoxine-p oxidase by destroying
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