한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제4권3호
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- Pages.105-110
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- 1976
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
농산폐자원의 미생물학적 이용에 관한 연구 (제오보) 섬유소분해효소 생산 곰팡이의 분리 및 선별
Studies on The Microbial Utilization of Agricultural Wastes (Part 5) Isolation and Selection of Cellulase Producing Fungi
- Bae, Moo (Applied Microbiology Lab., Korea Institute of Science and Technology) ;
- Kim, Byung-Hong (Applied Microbiology Lab., Korea Institute of Science and Technology) ;
- Lee, Gye-Jun (Applied Microbiology Lab., Korea Institute of Science and Technology)
- 발행 : 1976.09.01
초록
곰팡이를 이용하여 섬유소분해효소를 생산할 목적으로 전국각처에서 수집한 102종의 시료로부터 총 221주의 곰팡이를 분리하였다. 이들 분리균주의 섬유소분해능을 여과 지붕괴법 ? 액화법 및 cup method로서 1차 36균주를 선별하였고 1차 선별된 균주를 탄소원을 변경하여 배양한뒤 활성을 추정하여 2차로 15균주를 선별하였다. 15균주를 개선된 배지에서 배양한뒤
In the studies of microbiological utilization of cellulosic wastes, cellulolytic fungi were isolated and screened out. At the first stage, 221 cellulolytic fungi were isolated from different sources such as soils, humus, composts and rotten wood debris by enrichment culture techniques. In the second stage, 36 strains of fungi out of those previously isolated were selected for their cellulase activities estimated by means of filter paper degradation, carboxy methyl cellulose liquefaction and cup method. Activities of C
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