The Korean Journal of Zoology (한국동물학회지)
- Volume 19 Issue 1
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- Pages.1-6
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- 1976
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- 0440-2510(pISSN)
Glycogen Content in the Mouse Oocytes
생쥐난자의 Glycogen함량
- Yoon, Yong-Dal (Dept. of Zoology, Seoul National University) ;
- Cho, Wan-Koo (Dept. of Zoology, Seoul National University)
- Published : 1976.03.01
Abstract
The glycogen content of the oocytes at the various stages of meiotic division induced during culture was determined by a microspectrophotometer. The PAS intensity decreased gradually as the meiotic resumption progressed. The amount of glycongen was also decreased in the degenerated ova. Is is concluded that the glycogen consumption in necessary for the meiotic resumption and that the glycogen loss while the germinal vesicle is intact seems to lead degeneration. These results suggest that the endogenous glycogen is important to support meiosis.
생쥐 여포난자를 배양하면서 감수 분열 각 단계에서 난자내 glycogen함량의 변화를 Microspectrophotometer를 사용하여 조직화학적방법으로 조사하였다. 난자의 성숙이 진행됨에 따라 PAS반응은 감소하며, 퇴화중인 난자의 glycogen함량은 현저히 적었다. 난자내 glycogen은 난자의 성숙을 유도하는 역활을 나타내며, 핵막이 붕괴하기 전에 glycogen이 소모되면 난자의 퇴화를 일으킨다. 본 실험의 결과 난자내 glycogen은 감수분열의 진행에 있어서 중요한 요인이 됨을 알 수 있었다.
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