Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Production of Fermented Feeds from Agricultural Waste Products (Part Ⅲ) -On the Production of Cellulase by Aspergillus niger and Trichoderma viride-

농산폐기물(農産廢棄物)에서 발효사료(醱酵飼料)의 생산(生産)에 관(關)한 연구(硏究)[제3보(第三報)] -Aspergillus niger와 Trichoderma viride에 의(依)한 Cellulase의 생산성(生産性)에 관(關)하여-

  • Lee, Ke-Ho (Dept. of Food Technology, College of Agriculture, Seoul National University) ;
  • Koh, Jeong-Sam (Dept. of Food Technology, College of Agriculture, Seoul National University) ;
  • Park, Sung-O (Dept. of Food Technology, College of Agriculture, Seoul National University)
  • 이계호 (서울대학교 농과대학 식품공학과) ;
  • 고정삼 (서울대학교 농과대학 식품공학과) ;
  • 박성오 (서울대학교 농과대학 식품공학과)
  • Published : 1976.09.30
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Abstract

In order to utilize the agricultural waste products, two strains of mold producing powerful cellulolytic enzyme were sereened from various soils, composts, rotten wood and others. The optimum condition of cellulase production was studied. The results obtained were summarized as follows. 1. Two strains of mold which showed remarkably high cellulolytic activity were identified as Aspergillus niger-SM 6 and Trichoderma viride-SM 10. 2. The highest cellulase production was obtained at pH 5.0-6.0 in 5 days. 3. Cellulase production in strain Aspergillus niger-SM 6 increased with the addition of C.M.C., $(NH_4)_2SO_4$, C.S.L., orange peel powder and rice hull. The rice hull, treated with 3N NaOH at $120^{\circ}C$ for 15 min. and neutralized with various acids, was used. Up to 50% of wheat bran could be substituted by the treated rice hull without any decrease of cellulase activity. 4. In the strain of Trichoderma viride-SM 10, cellulase production increased with the addition of C.M.C., $NH_4NO_3$, Vitamin-free casamino acid and orange peel powder, while the other carbon, nitrogen, phosphate sources, natural nutrients and organic substances gave no remarkable effect.

농산페기물을 이용(利用)한 발효사료(醱酵飼料) 생산(生産)에 관한 기초적인 연구로서 25점(點)의 시료(試料)에서 cellulase 생산성(生産性)이 우수한 두개의 균주를 선정(選定)하여 동정(同定)하였다. 선정(選定)된 두 균주에 대하여 cellulase의 생산 최적조건을 검토하여 다음과 같은 결과를 얻었다. 1. Cellulase 생산성(生産性)이 우수한 균주(菌株)로 Aspergillus niger-SM6와 Trichoderma viride-SM10 두 균주를 선정(選定)하였다. 2. 두 균주 모두 배양시(培養時) pH $5.0{\sim}6.0$에서 배양(培養)5일째에 최고(最高)의 효소생산성(酵素生産性)을 나타내었다. 3. Aspergillus niger-SM6은 배양시(培養時) 첨가한 CMC가 18%, $(NH_4)_2SO_4$가 33%, CSL이 21%, 감귤피가 21%의 효소생산(酵素生産) 증가를 나타내었다. 또한 전처리한 왕겨를 첨가하여 배양하였을 때 $21{\sim}28%$ 효소생성이 증가하였으며 효소생산에 있어서 50%까지 밀기울과 대체가능하였다. 4. Trichoderma viride-SM10은 배양시 첨가한 CMC가 14%, $NH_4NO_3$가 40%, Vit-free casamino acid가 27%, 감귤피가 18%의 효소생산 증가를 나타내었다.

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