Studies on the Histological Observation of Removing the Skin from Squid by Various Treatments

각종처리(各種處理)에 의한 오징어의 박피(剝皮)에 관(關)한 조직학적관찰(組織學的觀察)

Treated with various methods, body muscle of squid was cross section, stained by Van Gieson staining method, and observed microscopically to study on the state of thin layer of skin separated from meat. Results obtained were summarized as follows : 1. By general hot spring treatment were 1st. 2nd. and 3rd. layers of skin removed from squid muscle but 4th. not. 2. By boiling treatment was tissue of muscle fibre fissured largely toward the muscle fibre. 3. Sodium acetate treatment was superior to sodium nitrate, sodium sulfate and sodium tartrate treatment in the effect that the skin was separated from meat. Especially, concentrate solution of sodium acetate had the most excellent effect in the chemical reagent treatment. 4. By proteolytic enzyme treatment were 1st. 2nd. 3rd. and 4th. layers of skin removed all from squid muscle and the boundary fibre between skin and meat swelled in particular. 5. Two kinds of skin removing method, proteolytic enzyme treatment and sodium acetate treatment, were desirable to the actual processing.

오징어의 동부근육(胴部筋肉)을 각종방법(各種方法)으로 처리(處理)하여 cross section하고 Van Gieson방법(方法)으로 염색(染色)하여 박막상(薄膜狀)의 표피층(表皮層)이 박리(剝離)되는 상태(狀態)를 현미경으로 관찰한 결과(結果)는 다음과 같다. 1. 일반적으로 사용되는 온탕법(溫湯法)에서 1, 2, 3층(層)은 제거(除去)되지만 4층(層)은 거의 제거(除去)되지 않았다. 2. 열탕처리(熱湯處理)에 의해서 근섬유(筋纖維) 조직(組織)에 근섬유(筋纖維)의 방향(方向)으로 많은 균열 현상을 보였다. 3. 초산소오다가 질산소다. 황산(黃酸)소오다 및 주석산(酒石酸)소오다 처리(處理)보다 박리상태(剝離狀態)가 현저히 우수하였으며 특(特)히 고농도(高濃度)에서 현저하고 약품처리(藥品處理) 방법중(方法中) 가장 우수한 효과(?果)를 보였다. 4. 단백(蛋白) 분해효소(分解酵素) 처리시(處理時) 표피(表皮)의 1, 2, 3, 4, 층(層)은 완전히 제거(除去)되며 특(特)히 표피(表皮)와 육(肉)과의 경계면 섬유(纖維)를 현저하게 팽윤(澎潤)시켰다. 5. 효소(酵素)와 초산소오다로 처리(處理)하는 2종류(種類)의 박피법(剝皮法)은 유효(有效)하다는 것을 확인(確認)하였다.