Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Study on Stable Fly Eradication by Sterile-male Technique (4) Effects of X-ray Irradiation on the Stable Fly

웅성불임기술을 이용한 쇠파리 구제에 관한 연구 (4) 방사선 조사가 쇠파리에 미치는 영향

  • Chung K. H. (Applied Genetics Lab., Korea Atomic Energy Research Institute) ;
  • Ryu J. (Applied Genetics Lab., Korea Atomic Energy Research Institute) ;
  • Kwon S. H. (Applied Genetics Lab., Korea Atomic Energy Research Institute)
  • 정규회 (한국원자력연구소 응용유전학연구실) ;
  • 유준 (한국원자력연구소 응용유전학연구실) ;
  • 권신한 (한국원자력연구소 응용유전학연구실)
  • Published : 1975.10.01
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Abstract

This experiment was performed to investigate the X-ray sensitivities at the various stages of life cycle and to determine the sterillizing dose of stable fly, Stomoxys calcitrans(L). A dose of 300 rad caused about $50\%$ mortality in 2-hour-old eggs as measured by egg hatch, and $100\%$ mortality was obtained with a does of 1 Krad. Sub-lethal dose $(LD_{50})$ for the pupal mortality after irradiation of 3-5days old pupae were 11.5 Krad and the mortality was decreased with increasing pupal age at irradition. A significant reduction of egg hatch by $1.5\%$ was observed when treated males with 3 Krad at pupal stage were mated to untreated virgin femleas. On the other hand, $100\%$ sterility in females was resulted by 2 Krad irradiation and oviposition was completely inhibited with 3 Krad. Thus, both sexes of stable fly could be sterilized with a dose of 4 Krad irradiated to 3-5 days old pupae.

쇠파리의 난과 3-5일령 및 7-9일령 용에 X-선을 조사시켜 부화율, 우화율을 조사하였으며 또한 3-5일 영용에 X-선을 조사시켜 우화된 성충의 불임유기선량에 대한 실험결과를 다음과 같이 얻었다. 1. 란부화율은 약 300R 조사시 $50\%$이었으며 3-5일영 및 7-9일영 번데기에 대한 사충율의 회귀직선은 Y=2.665x+2.301, Y=4.36x_0,18이고 중앙치사선량$(LD_{50})$은 11.5KR과 12.6KR이었다. 2. 3KR 조사웅충과 정상자충에서 산란된 란은 약 $98\%$가 미부화되었다. 그러나 2.KR 조사자충과 정상웅충 사이에서 산란된 란은 $100\%$가 부화되지 않았다. 3. 불임유기선량은 자충이나 웅충과 같이 4KR이면 완전할 것으로 보며 $(LD_{50})$선량을 고려할 때 충분히 이용가능할 것으로 본다.

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