Studies on the Anthocyanins in Wild Vines (Vitis amurensis Ruprecht). - (Part 1) Separation and Determination of Anthocyanins in Wild Vines -

머루(Vitis amurnesis Ruprecht) Anthocyanin에 관(關)한 연구(硏究) - [제 1 보(第 1 報)] 머루 Anthocyanin의 분리(分離) 및 정량(定量) -

The content of total and individual anthocyanins in the wild vines (Vitis amurensis Ruprecht), which were wildly grown in the mountain area of Korea, has been carried out. The pigments were extracted with 0.1% hydrochloric acid in methanol from the wild vines and the content of the total anthocyanin in the extract was determined spectrophotometrically at 538nm $({\lambda}max.)$. Individual anthocyanins in the extract were separated by paper chromatography and eluted with 0.1% HCl in methanol. The optical densities of the individual pigment solutions were determined at each absorption maxima of the pigments. The content of total anthocyanins in wild vines was 3.95mg per 1g fresh weight. The content of individual pigments were as follows: 12.5% delphinidin 3-monoglucoside. 3,5 % petunidin 3-monoglucoside, 2.1% cyanidin 3-monoglucoside, 10.1% delphinidin 3,5-diglucoside, 4.3% malvidin 3-monoglucoside, 8.3% petunidin 3,5-diglucoside, 4.1% cyanidin 3,5-diglucoside like and 55.1% malvidin 3,5-diglucoside. It was found that the most abundant pigment in wild vines studied was malvidin-3,5-diglucoside.

우리나라 산간지방(山間地方)에 야생(野生)하는 머루 중(中)의 총(總) anthocyanin의 함량(含量)과 개별(個別) anthocyanin의 조성(組成)을 측정(測定)하였다. 머루로 부터 색소(色素)를 0.1% HCl methanol로 추출(抽出)하여 파장(波長) 538nm $({\lambda}max.)$에서 흡광도(吸光度)를 측정(測定)하여 총(總) anthocyanin 함량(含量)을 구(求)하였다. 추출액중(抽出液中)의 개별(個別) anthocyanin들은 paper chromatography로 분리(分離)하고 0.1% HCl methanol로 용출(溶出)하였다. 분리(分離)된 개별(個別) anthocyanin들의 용액(溶液)을 各己의 흡수극대(吸收極大) 파장(波長)에서 흡광도(吸光度)를 측정(測定)하여 조성비(組成比)를 구(求)하였다. 머루 중의 총(總) anthocyanin 함량(含量)은 신선물(新鮮物)의 g 당(當) 3.95mg이였다. 개별(個別) anthocyanin들의 조성비율(組成比率)은 다음과 같다. delphinidin-3-monoglucoside 12.5%, petunidin-3-monoglucoside 3.5%, cyanidin-3-monoglucoside 2.1%, delphinidin-3,5-diglucoside 10.1%, malvidin-3-monoglucoside 4.3%, petunidin-3,5-diglucoside 8.3%, cyanidin-3,5-diglucoside like 4.1%, malvidin-3,5-diglucoside 55.1%. 머루 중의 주(主) 색소(色素)는 malvidin-3,5-diglucoside이였다.