Studies on the Hydrolysis of Inulin in Jerusalem Artichokes by Fungal Inulase

미생물(微生物) Inulase에 의(依)한 돼지감자 중의 Inulin분해(分解)에 관한 연구(硏究)

The analysis of Jerusalem artichoke showed that it contains 12.09% of Inulin. The results obtained from the examination of the conditions for fructose production by cultivating Pencillum sp 1 in the Jerusalem articoke medium were as follows: 1. The optimum amount of water added to Jerusalem artichoke was 2.5 $\ell$ of distilled water per ㎏ of fresh Jerusalem artichoke. It this case, the concentration of Inulin was 4% (w/v). 2. The optimum temperature was $30^{\circ}C$, the initial optimum pH was 5.0 and the optimum cultural period was 72 hours. 3. Shaking culture with 50 ml of the medium and 120 oscills/min in 500 ml shaking flask was most effective as the culture method. 4. 0.1% of $NH_4H_2PO_4$ as a nitrogen source, 0.001 of $FeSO_47H_20$ and 0.001% of $MgSO_47H_2$ as metal salts were most effective. 5. Fructose production continued to increase for 72 hours under the optimum conditions for cultivation and the highest production rate to the Inulin was 95.25%.

돼지감자를 분석(分析)하여 탄수화합물(炭水化合物)로써 존재(存在)하고있는 Inulin성분량(量)이 12.09% 함유(含有)하고 있음을 확인(確認)하였다. pencillium sp 1을 돼지감자배지(培地)에 배양(培養)하여 과당생산조건(果糖生産條件)을 검토(檢討)한 결과(結果)는 다음과 같다. 1. 돼지감자에 대(對)한 최적가수량(最適加水量)은 신선돼지감자 1kg에 대(對)하여 증류수(蒸溜水) 2.5$\ell$이다. 이때의 Inulin 농도(濃度)는 4%(W/V)이였다. 2. opt. temp는 $30^{\circ}C$, opt pH는 Initial pH로 5.0, opt cultcoral period 는 72 hrs 이였다. 3. 배양방법(培養方法)은 500ml shaking flask 에 배지량(培地量)을 50ml로 하고 120oseills/min 으로 shaking culture 가 가장 효과적(?果的)이었다. 4. 질소원(窒素源)으로써는 $NH_4H_2PO_40.1%$ 금속염(金屬鹽)으로써는 $FeSO_47H_2O$ 0.001%, $MgSO_47H_2O$ 0.001%가 가장 효과적(效果的)이었다. 5. 최적배양조건하(最適培養條件下)에서 과당생성(果糖生成)은 72 hrs 까지 계속증가(增加)되었고 최고(最高)의 생산량(生産量)은 95.25%이었다.