Comparative Studies on Short Term Blood Culture Method for Bovine Chromosome Investigation

소의 염색체 검사를 위한 혈액배양방법의 비교연구

The studies were carried on to set up a short term blood culture method which is essential for doing bovine cytogenetic investigation and to reduce the amount of the various reagents wish better results obtained with modified methods were compared with the results of Basrur's and Halnan's methods, and the following results were obtained. 1. The modified method, with lesser amount of reagents than other methods, produced better results and showed cleaner spreads. 2. Better spreads were obtained, in the modified method, by modifying the hypotonic treatment. 3. Reduction of the PHA to 1/10 comparison with the other methods showed no harmful effects.