대한미생물학회지 (The Journal of the Korean Society for Microbiology)

대한미생물학회 (The Korea Society for Microbiology)
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Crocin첨가 결핵균 배지상의 색소형성에 의한 비정형 Mycobacteria의 분류

Subdivision of Opportunist Mycobacteria by the Difference of Pigment Production on Lowenstein-Jensen Medium Containing Crocin

  • 최철순 (가축위생연구소 세균과) ;
  • 김재학 (가축위생연구소 세균과) ;
  • 윤용덕 (가축위생연구소 세균과) ;
  • 이현수 (가축위생연구소 세균과) ;
  • 이택주 (가축위생연구소 세균과)
  • Choi, Chul-Soon (Bacteriology Division, Institute of Veterinary Research) ;
  • Kim, Jae-Hak (Bacteriology Division, Institute of Veterinary Research) ;
  • Yoon, Yong-Dhuk (Bacteriology Division, Institute of Veterinary Research) ;
  • Lee, Hyun-Soo (Bacteriology Division, Institute of Veterinary Research) ;
  • Lee, Taek-Chu (Bacteriology Division, Institute of Veterinary Research)
  • 발행 : 1974.12.31
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초록

Mycobacteria의 Crocin 첨가 결핵균 배지상의 보라색 색소형성과 광선조사, 호혐기상태, Crocin의 농도 및 배양시간과의 관계를 조사하고 비정형 Mycobactia의 색소형성 특성에 따른 군분류 동정에 대하여 기술하였다. 광색정군 M. kansasii는 양성으로 음성인 M. marinum과 감별이 되었으며, 색정군 M. scrofulaceum은 음성으로서 양성인 M. aquae와, 조기발육군 M. fortuitum은 3일 배양검사에서 양성으로 M. smegmatis로 부터 본류 동정이 각각 가능하였다.

On the basis of pigment production and growth rate on L-J medium containing crocin, differentiation of opportunist mycobacteria belonging to photochromogens, scotochromogens, nonchromogens and rapid grower has been investigated. Among photochromgens, positive pigmentation of M. kansasii was differentiated from negative strain of M. marinum, Scotochromogen M. aquae was positive whereas M. scrofulaceum was negative. Rapid grower M. fortuitum was positive at 3 days test whereas M. smegmatis was negative. Subdivision of opportunist mycobacteria into four groups on the basis of growth rate and pigment production on L J medium containing gardenia extraction appeared to be a valuable adjunct to the Runyon's classification for the rapid presumptive identification of opportunist mycobacteria of different clinical significance.

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