A Study on the Isolative Determination of 4-Morpholine Carboximidoylguanidine Hydrochloride in Ophthalmic Solutions

점안제중(點眼劑中) 4-Morpholine Carboximidoylguanidine Hydrochloride의 분리정량(分離定量)

The isolative determination of 4-Morpholine carboximidoylguanidine Hydrochloride in the ophthalmic solutions was examined by means of colorimetric method using oxidized nitroprusside and sodium hypobromite. According to the results, the following could be summerzied. 1) The wave length of maximun absorption in $650\;m{\mu}$. 2) Beer's Lew is obeyed for $20{\sim}80\;mcg/ml$ in colored solutions. 3) Absorptions of colored solutions must be measured 5 minutes after the addition of oxidized and 5 minutes after the completion of sodium hypobromite. 4) 4-Morpholine carboximidoylguanidine hydrochloride can be determined within standard deviation 2.0%.