Studies on the Cellulase of Penicillium sp. Isolated from Soils - (II) Culture Conditions of Penicillium sp. C13-13 Strain -

토양에서 분리한 Penicillium sp.가 생산하는 Cellulase에 관한 연구 - (II) Penicillium sp. C13-13 주(株)의 배양조건 검토 -

1. Penicillium sp. C 13-13 strain was obtained with the treatment of mutagenic agents(N.T.G.) and by single spore isolation method from the Penicillium sp. C8-14 strain, which was reported in the previous paper. 2. The above strain had a few spores and to obtain seed culture, it was cultured at $30^{\circ}C$ and initial pH $4.5{\sim}5.0$, with air rate 6l/min., and agitation 600 rpm for 48 hours in 10% wheat bran medium in 20l- Jar fermenter. When the broth that had above 70ml of mycelium was inoculated into wheat bran medium and incubated at $29{\sim}33^{\circ}C$ for 72 hrs, the cellulase activity of the koji was higher. 3. Adding calcium chloride and magnecium sulfate to the wheat bran medium to 1.5% and 0.015% respectively, the cellulase activity of the koji was higher than that of the control.

Penicillium sp. C8-14주(株)를 계속 단포자 분리 및 변이 처리를 하여 Penicillium sp. C13-13(역가-3171u/g) 주(株)를 얻었다. 2. 고체배양에 적용하기 위하여 증식용 액체배양을 하였으며, 초발 pH $4.5{\sim}5.0$, 10% 부배지를 사용하여 48시간동안 $30^{\circ}C$, 통기량 6l/min, 교반속도 600rpm(20l-Jar fermenter)으로 배양하여 균체량이 70ml이상일 경우에 접종한 것이 $29{\sim}33^{\circ}C$, 72시간의 부배지 배양에 있어서 가장 좋은 역가를 보였다. 3. 부배지에 1.5% $CaCI_2$ 및 0.015% $MgSO_4$를 가한 것이 control 보다 높은 역가를 보였다. 끝으로 본고(本稿)작성에 많은 도움을 주신 서울 대학교 약학대학 김병각 교수님께 심심한 사의를 표합니다.