The Journal of the Korean dental association (대한치과의사협회지)
- Volume 11 Issue 8
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- Pages.525-530
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- 1973
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- 0376-4672(pISSN)
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- 2713-7961(eISSN)
Collagenolytic Activity of Solid Tawa Sarcoma
결절형 Tawa육종의 Collagenase에 관한 연구
- Chung, Tai-Young (Dept. of Biochemistry College of Dentistry S.N.U.) ;
- Sakaki, Tetsuya (Dept. of Biochemistry Osaka Dental University) ;
- Tawa, Toshikazu (Dept. of Biochemistry Osaka Dental University)
- Published : 1973.08.30
Abstract
True collagenolytic enzymes in animal tissues were first demonstrated by Gross and Lapiere (1962), who showed the ability of such an enzyme in the culture medium of living explants of tadpole tissue to degrade a specific substrate of undenatured collagen under physiological conditions. Recently, tumor-associated collagenolytic activity has been demonstrated in human neoplasm and in ascites V Carcinoma. This investigation have been peforme to determine whether or not a collagen lytic enzyme could e found in isolated solid Tawa sarcoma of Donryu female rat obtained the culture medium. The results were as follows. 1. 11.5mg% of hydroxyproline contained in Donryu rat skin collagen, which was extracted by 0.5M acetic acid. 2. Cultivation of solid Tawa sarcoma tissues on reconstituted rat skin collagen gels showed lysis of adjacent gel after 18 hours, and much more extensive lysis after 5 days. 3. Collagen substrate was not attacked by the common proteolytic enzymes, trypsin, pepsin, and pronase.
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