Effect of the Dietary Composition (quantitative ratio between carbohydrate and protein) and the Rearing Temperature during the 4th Moulting Period on Amylase activity of Digestive Juice in the Silkworm, Bombyx mori L.

가잠유충의 소화액 Amylase 활성에 미치는 사료조성(당과 단백질과의 량비) 및 4면기보호온도의 영향

his study has been carried out to investigate amylase activity of digestive juice in the 5th day of the 5th instar influenced by the dietary composition (quantitative ratio between carbohydrate and protein) and the rearing temperature during the 4th moulting period. The larvae grew on three kinds of semi-synthetic diets. The A-diet has more carbohydrate than the others, the B-diet has carbohydrate in 1 : 2 with protein, and the C-diet has more protein than the others. All the diets were kept at 16$^{\circ}C$, 25$^{\circ}C$ and 32$^{\circ}C$ during the 4th moulting period. Amylase activity of digestive juice at the 5th day of the 5th instar was analyzed by Fuwa's method. The results were as follows. 1. Both A and C-diets were worse than B-diet in the larval growth and development. 2. The dietary composition influencing amylase activity of digestive juice was not related to the rearing temperature during the 4th moulting period. Amylase activity was stronger in C-diet, B-diet and A-diet order. 3. It was found that amylase activity at 32$^{\circ}C$ was stronger than that at 16$^{\circ}C$ in all kinds of diets. 4. There was an inter-action in amylase activity of male larval digestive juice between the dietary composition and the rearing temperature during 4th the moulting period.

누에의 소화액 Amylase 활성에 미치는 영양 및 환경의 영향을 알기 위하여 사료조성중에 당의 함량이 많은 A사료(따라서 단백질의 함량은 적음), 당과 단백질과의 량비가 약 1 : 2로 적당하다고 생각되는 B사료, 단백질의 함량이 많은 C사료(따라서 당의 함량이 적음)의 3종의 준합성사료를 사용하여 유충을 사육하고, 4 면기를 16$^{\circ}C$, $25^{\circ}C$, 32$^{\circ}C$로 각각 보호한 5령 5일째 유충의 소화액 Amylase 활성을 조사하였던바 다음과 같은 결과를 얻었다. 1. 유충의 성육은 B사료보다 A사료나 C사료에 있어서 다같이 불량하였다. 2. 유충의 소화액 Amylase 활성은 4 면기 보호온도와 상관하지 않고 C사료＞ B사료＞ A사료의 순으로 강하였다. 3. 유충의 소화액 Amylase 활성은 A.B.C의 각 사료에 있어서 대개 같은 경향으로서 32$^{\circ}C$보다 16$^{\circ}C$에 있어서 강하였다. 4. 유충의 소화액 Amylase 활성에미치는 사료조성과 4 면기 보호온도와의 교호작용은 자에선 인정되지 않았으나 웅에 있어선 인정되었다.