초록
1) 탄소원으로서 glucose medium에서 Brevibacterium flavum을 배양할때 Biotin 5${\gamma}$/1, Desthiobiotin 5${\gamma}$/1, 7.8-Diaminopelargonic acid 10${\gamma}$/l 에서 L-GA생성이 가장 양호하였다. 2) 탄소원으로서 glucose-acetate혼합배지를 4구분하여 사용한 결과 생육도, L-GA생성은glucose 2%-acetate 혼합배지가 가장 양호하였다. 3) 탄소원으로서 Methanol, Ethanol, Ethylacetate, Acetic acid (free acetate), Na-acetate :NH$_4$-acetate=2 : 1의 배지를 사용한 결과 Na-acetate : NH$_4$-acetate=2 : 1 > Acetic acid (free acetate) > Ethylacetate > Ethanol> Methanol 의 순으로 L-GA 생성이 나타났다. 4) 생장촉진제로 각종 Biotin vitamer를 첨가하고 탄소원으로 glucose 또는 Acetate 배지를 사용하였을때 요구되는 농도는 Biotin에 대하여 Desthiobiotin은 대략 동양, 7.8-Diaminopelargonic acid는 3~4배 였으며 Bisnorbiotin은 대체효과가 없었다.
The effect of biotin and biotin vitamer on the fermentative production of L-glutamic acid (L-GA) by Brevibacterium flavum was studied. And results were as follows. 1) L-GA production in the medium containing 10％ Glucose was the best at the concentration of Biotin 5${\gamma}$/l, Desthiobiotin 5${\gamma}$/1, and 7,8-Diaminopelargonic acid 10${\gamma}$/1, respectively. 2) In the experiment using the Glucose-Acetate mixed media derided into four parts, considerable amounts of cell growth and L-GA production were observed in the mixed medium containing 2％ Glucose-Acetate. 3) In the cases of using the media containing methanol, ethanol, ethylacetate, acetic acid (free acetate), Na-acetate:NH$_4$-acetate=2 : 1, the production of L-GA were in decreasing order as follows; Na-Acetate:NH-Acetate=2 : 1> Acetic acid (free acetate)> Ethylacetate> Ethanol＞ Methanol. 4) When biotin vitamers as growth factors were added in the medium containing Glucose or Acetate as the source of carbon, the substitution effect of Desthiobiotin was almost the same, 7,8-Diaminopelargonic acid 3 or 4 times stronger, and Bisnorbiotin has no substitution effect, compared with Biotin.
