Stuidies on Production of Nucleic acid Derivatives by Microorganisms (IV) -Effect of Culture Media on 5′-IMP Accumulation by Brevibacterium ammoniagenes-

미생물에 의한 핵산관연물질의 생산에 관한 연구(제사보) -Brevibacterium 속 세균 변리주의 5'-inosinic acid 발효 배지성분에 관하여 -

  • Bae, Moo (Applied Microbiology Lab., Korea Institute of Science and Technalogy) ;
  • Lee, Gye-Jun (Applied Microbiology Lab., Korea Institute of Science and Technalogy)
  • 배무 (한국과학기술연구원.응용미생물연구실) ;
  • 이계준 (한국과학기술연구원.응용미생물연구실)
  • Published : 1973.12.01

Abstract

Effects of nitrogen sources, supplement of vitamins and minerals on the accumulation of 5'-inosinic acid by an adenineless mutant of Brevibacterium ammoniagenes were examined. Maximal yield of 5'-inosinic acid was attained by the use of yeast extract. as organic nitrogen source Casamino acid supplemented with $Mn^{++}$, $Zn^{++}$ Ca-D-pantothenate and thiamine HCl could be subsitute for it. Subsequent experiment using the defined medium showed that the concentration of these trace element in the medium affected inosinic acid accumulation markedly. And it was found that the simulataneous addition of $Mn^{++}$ (20$\mu\textrm{g}$1). $Zn^{++}$(10$\mu\textrm{g}$1). thiamine Hcl(10mg/1) and Ca-D-pantothenate (5mg/l) to the defined medium stimulated inosinic acid accumulation, from which 7mg/ml of inosinic acid was obtained.

핵산관연물질중 정미성물질인 5'-inosinic acid을 직접발효법으로 생산함에 있어 배지조성의 검토를 본 실험에서 수행하였다. 유기질소원으로서는 yeast extract 1%을 사용함이 좋았고 biotin의 첨가량은 30 $\mu\textrm{g}$l였을때 IMP의 축적량이 가장 많았다. 유기질소원으로 Casamino acid를 쓸 경우 $Mn^{++}$ 20$\mu\textrm{g}$1. $Zn^++/10$\mu\textrm{g}$1, Thiamine HCl 10mg/1 및 Ca-D-pantothenate 5 mg/l를 첨가해주면 IMP의 축적량이 증가하였으며 최고 7.0mg/ml의 IMP가 발효배지중에 생성되었다.었다.

Keywords