Abstract
For efficient micro scale syntheses of Rose $Bengal-^{131}I$, $Hippuran-^{131}I$, and $H.S.A.-^{131}I$, the dependence of labelling yields on pH, on salt contents, and on the volume of buffer solution in the reaction mixtures as well as the reaction apparatus were studied. pH of 5.6 was optimum for preparation of both Rose $Bengal-_{131}I$ and Hippuran $-^{13}I$ but pH of 8.5 was optimum for preparation of $H.S.A.-^{131}I$. Salt in the reaction mixtures hindered drastically the formation of $Hippuran-^{131}I$ but it slightly increased the labelling yield of H.S.A.. The compactly closed reaction vessels were effective for preparations of both Rose $Bengal-^{131}I$and $Hippuran-^{131}I$ in small volume. Thereupon, the labelling procedures were modified to bring about higher labelling yields and better reproducibilities. By these newly established procedures, the labelling yields of Rose $Bengal-^{131}I$ and $Hippuran-^{131}I$ could be increased even with the home-produced sodium $iodide-^{131}I$ solution containing reducing agent.
Rose $Bengal-^{131}$ /I, $Hippuran-^{131}$ /I, $H.S.A-^{131}$ /I 등을 효과적으로 합성하기 위해 표지 반응액의 pH, 염의함량, 반응액중의 완충용액의 부피 및 합성장치등에 따르는 표지 반응수율을 검토하였다. Rose $Bengal-^131{ }$I 및 $Hippuran-^{131}$ /I 의 반응수율은 PH 5.6에서, $H.S.A-^{131}$ /I 반응수율은 pH 8.5에서 각각 가장 좋았다. 반응액중에 함유된 염은 $Hippuran-^{131}$ /I의 생성반응을 크게 저해 시켰으며 H.S.A.의 표지수율은 어느 범위안에서 오히려 약간 향상시켰다. Rose $Bengal-^{131}$ /I 나 $Hippuran-^{131}$ /I 를 소규모 합성할 경우는 밀폐된 용기가 효과적이었다. 이상의 결과에 따라 더 높은 표지수율과 좋은 재현성을 얻을 수 있는 반응조건을 확립하였으며 이에 따라 환원제가 함유된 국산 $Na^{131}$ /I를 사용하더라도 Rose $Bengal-^{131}$ /I과 $Hippurn=^{131}$ /I의 표지수율을 높일 수 있었다.