Histochemistry of the Developing Chick Respiratory Tract

鷄胎呼吸氣道의 組織化學的 硏究

A histochemical study of the respiratory tract in developing chick was done to demonstrate PAS-postivie materials, ribonucleic acid, phospholipid, and alkaline phosphatase activity. Following results are obtained: 1. The alkaline phosphatase activity was found to be high before the appearance of cartilage in mesenchyme surrounding the tracheal epithelium. The enzyme activity declined after the cartilage formation, followed by the restricted activity in epithelium in the postembryonic stage. 2. A moderate positive reaction of ribonucleic acid was found in the cytoplasm of the epithelium and undifferentiated mesenchyme. As the cartilage grew differentiated the reaction of ribonucleic acid was found to disappear in the mesenchyme surrounding the epithelim, but the cytoplasm of the glands showed a moderate positive reaction. 3. Goblet cells of the mucosa and glandular cells showed highly positive reaction, but the basement membrane exhibited slightly positive PAS-reaction. 4. Epithelial cells of the mucosa showed a weak to moderate reaction. However, the epithelia of bronchiol and alveoli in the differentiating period and glandular cells showed a strong positive reaction in Baker's hematein test.

저자는 白色레그혼의 種卵을 孵化시켜 얻은 鷄胎呼吸氣道의 alkaline phosphatase, RNA, PAS 陽性物質 및 燐肥質의 분포와 소장을 組織化學的으로 관찰하여 다음과 같은 결과를 얻었다. 1. alkaline phosphatase는 軟骨形成에 앞서 氣道上皮 주위의 間充織에 고도의 活性을 나타냈으며 軟骨形成 後에는 反應은 감소되었고 胎生後期에는 phosphatase 活性은 上皮에만 관찰되었다. 2. RNA는 胎生초에 氣道上의 細胞質內에 陽性反應을 보였으며 軟骨形成에 앞서 上皮주위에 밀집하기 시작한 間充織에도 점차 陽性을 나타냈으나 軟骨形成후에는 이 反應은 감소하였고 鷄胎末期에 腺形成에 따라 腺上皮의 細胞質內에도 陽性 呈色反應을 보엿다. 3. PAS 陽性物質은 基底膜, 杯狀細胞 및 腺形成後에 腺內腔을 제외하고는 陰性反應을 나타냈으며 燐脂質은 發生期의 肺域내의 細氣管支上皮와 腺胞를 제외하고는 비교적 약한 呈色反應이나 陰性을 보였다.