Applied Biological Chemistry
- Volume 14 Issue 1
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- Pages.1-7
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- 1971
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
A Study on the Development of A Juice-clarifying Enzyme Preparation
과실쥬우스용 효소제의 개발연구
- Lee, Su-Rae (College of Agriculture, Seoul National University) ;
- Chang, Kyung-Jung (College of Agriculture, Seoul National University)
- Published : 1971.04.30
Abstract
In order to develop an enzyme preparation for clarification of fruit juices, a microbial strain having a strong pectolytic activity was selected and a crude enzyme preparation from this strain was examined for the effects in the preparation of grape juice and wine. The results are summarized as follows: 1) A strain of Aspergillus niger was selected as having the highest productivity of pectolytic enzymes among many species of Aspergillus and Rhizopus. 2) A pectolytic enzyme preparation was purified from this selected strain and the effects of pH and temperature on its enzyme activity and stability were investigated. 3) The use of the enzyme preparation brought about the increase in the free run yield and clarity of grape juice. 4) Whereas the use of the enzyme preparation did not exhibit any effect in the brewing of red wine, its use showed a good effect on the rates of filtration and clarity in the case of white wine.
과실쥬우스용 효소제를 개발할 목적으로 펙틴분해력이 강한 미생물균주를 선택하고 이에서 얻은 효소제에 의하여 포도쥬우스 및 포도주 제조과정에 있어서의 효과를 시험하여 다음과 같은 결과를 얻었다. 1) Aspergillus 및 Rhizopus속 균주에서 펙틴분해효소 생산력이 가장 강한 Aspergillis niger를 선정 하였다. 2) 이 균주에 의한 효소의 생성과정과 정제과정을 실험하고 조효소제품의 활성과 안정도에 미치는 pH 및 온도의 영향을 조사하였다. 3) 포도쥬우스 제조시에 효소제를 사용하면 free run juice의 수율이 증가하고 투명한 쥬우스를 얻을 수 있었다. 4) 포도주 양조시에 효소제를 사용하면 적포도주에는 아무 효과를 가지지 못하나 백포도주의 경우에는 과즙의 여과촉진 및 청정효과가 있을것으로 기대된다.
Keywords