Abstract
The induction of polyhedroses in the silkworm, Bambyx mari L., was investigated treating the 5th instar larvae just after eodysis with high temperature (hot water bath at 40$^{\circ}C$ for 5 minutes or dry heat shock at 40$^{\circ}C$ for 30 minutes) and low temperature (5$^{\circ}C$ for 24 hours). The results obtained were as follows; 1. Comparing between the frequency of nuclear and cytoplasmic polyhedroses induced by cold and heat treatments (hot water bath at 40$^{\circ}C$ for 5 minutes), the induction ratio of the former is clearly less than that of the latter. But if the larvae tested with cold were left at room temperature (25$^{\circ}C$) for 30-120 minutes till the next hot water bath (40$^{\circ}C$) for 5 minutes and water bath (20$^{\circ}C$) for 5 minutes, treatments, the frequency of induced cytoplasmic polyhedrosis was more than that in the case of cold or hot water bath treatment alone. 2. The frequency of nuclear and cytoplasmic polyhedrosis induced by cold and successive heat (dry heat shock at 40$^{\circ}C$ for 30 minutes), left at room temperature (25$^{\circ}C$) ti11 the second treatment, the frequency of nuclear polyhedrosis was less than that of cytoplasmic polyhedrosis. 3. The reaction of nuclear polyhedra to stains also differs sharply from that of the cytoplasmic type. In a smear of nuclear polyhedra on a slide staining with Giemsa solution remains unstained against a stained back ground, in contrast to this, the cytoplasmic polyhedra take up stain readly.
가잠의 Virus다각체의 저온 및 고온처리에 대한 유발현상을 조사하기 위하여 5령기잠을 공시하고 저온(5$^{\circ}C$, 24hrs.), 고온(Hot water bath 에 4$0^{\circ}C$, 5min으로 침지 및 Oven 내에 4$0^{\circ}C$, 30min.으로 단독 및 복합처리하여 핵다각체병(N) 중장형 다각체병(C), 병발형(NC) 유발률을 조사하고 동시에 핵다각체병(N)과 중장형다각체병(C)대하여 Giemsa solution 염색법에 의한 염색성을 조사하였다. 1. 냉장(5$^{\circ}C$, 24 시간) 처리와 고온(Hot water bath, 4$0^{\circ}C$, 5분간) 처리를 한 핵복다각체병(N)은 중장형다각체병(C)보다 그 유발률이 적고 또 복합처리에 있어서도 중장형다각테병(C)의 유발율은 핵다각체병(N)보다 많다. 2. 냉장처리후 고온(4$0^{\circ}C$, 30분간 건열) 처리, 반대로 고온처리후 냉장처리한 Virus다각체의 유발률도 중장형다각체병(C) 보다 핵형다각예병(N)의 유발현상이 현저하게 많이 나타났다. 3. Giemsa solution 염색법에 의한 핵다각체병(N)의 다각체는 염색이 되지 않으나 중장형다각예병(C)의 다각체는 잘 염색되어 염색성에 의하여 구별할 수 있었다.