Applied Biological Chemistry
- 제12권
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- Pages.69-74
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- 1969
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) (제4보(第四報)) -효소학적(酵素學的) 성질(性質)및 효소생성능(酵素生成能)에 대(對)하여-
Studies on the glucose isomerizing enzyme. (Part IV) -On the enzymatic properties and treating conditions for high activity containing cells-
- Rhee, In-Koo (Department of applied microbiology, Agriculture college, Kyung-Pook National University) ;
- Seu, Jung-Hwn (Department of applied microbiology, Agriculture college, Kyung-Pook National University)
- 발행 : 1969.09.15
초록
본(本) 실험(實驗)에 사용(使用)한 공시균(供試菌)인 방선균류(放線菌類)의 K-17균주(菌株)가 생성(生咸)하는 이성화(異性化) 효소(酵素)가 작용시(作用時) 효소학적(酵素學的) 성질(性質) 몇 가지와, Xylose를 첨가(添加)하지 않은 배지상(培地上)에서 얻은 Enzyme Activity 가 낮은 생균체(生菌體)를 Xylose 용액(溶液)에 처리(處理)하므로써 Enzyme Activity가 높은 생균체(生菌體)를 얻을 수 있었다. 이들의 결과(結果)를 요약(要約)하면 1. 효소작용시(酵素作用時)
This glucose isomerizing enzyme, which Actinomyces sp. (strain, K-17) produced, was inhibited by citrate, oxalate, ethylene diamine tetraacetic acid and cysteine on the enzyme reaction. This enzyme isomerized xylose to ketose as well as glucose. The Michaelis constant of this enzyme was
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