Studies on Cellulase -Part 1. Isolation of Cellulase Forming Microorganisms and the Properties of Crude Enzymes-

Cellulase에 관(關)한 연구(硏究) -(제1보(第一報)) Cellulase 생성균(生成菌)의 분리(分離)와 조효소(粗酵素)의 제성질(諸性質)-

Out of some 400 strains of Microorganisms, cellulase forming organisms was isolated from night soil during the course of screening tests. Two strains, Ku-3371 and Ku-4383 were found capable of producing cellulase in the shaking culture. General properties of the crude enzyme were as the following results. 1. The optimum pH values on CMC-saccharifying, CMC-liquefying and filter paper disintegrating activities were 4.0 to 5.5. 2. The stable pH range was within 3.5 to 6.5, 3. The optimum temperature was $40-45^{\circ}C$, the thermal stability was below $50^{\circ}C$ except on paper disintegrating activity and completely inactivated at $70^{\circ}C$. 4. Dialyzed crude enzyme was activated by $Mn^{2+}\;and\;Co^{2+}$ repectively but $Hg^{2+}$ was strong inhibitor.

분뇨처리장(糞尿處理場)에서 400여(餘) 균주(菌株)를 분리(分離)한 결과(結果) 그중(中) 두 균주(菌株)가 cellulase생성(生成)이 강(强)하였다. 이 균주(菌株)는 진탕배양(振湯培養)으로 cellulase를 많이 생성(生成)하였으며 이들 효소(酵素)의 성질(性質)은 다음과 같다. 1. 최적(最適) pH는 4.0에서 5.5 범위이다. 2. 안전(安全) pH는 3.5에서 6.5 범위이다. 3. 최적온도(最適溫度)는 $40^{\circ}C$에서 $45^{\circ}C$ 이다. 4. 열안전성(熱安全性)은 $50^{\circ}C$ 이하이며 $70^{\circ}C$에서 거의 불활성화(不活性化) 된다. 5. 투석효소(透析酵素)는 $Mn^{2+}\;Co^{2+}$로 활성화되며 $Hg^{2+}$는 강한 조해제(阻害劑)이다.