Applied Biological Chemistry

  • 제11권
  • /
  • Pages.49-53
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  • 1969
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  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) -제2보(第二報); 분리선정(分離選定)된 균주효소(菌株酵素)의 성질(性質)에 대(對)해서-

Studies on the Glucose Isomerizing Enzyme -Part II. Some Properties of the Glucose Isomerizing Enzyme of K-17 strain-

  • Seu, J.H. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kim, C.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Ki, W.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Rhee, I.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kwon, T.J. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Woo, D.L. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University)
  • 발행 : 1969.04.30
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초록

본(本) 연구(硏究)에서 얻어진 결과(結果)를 요약(要約)하면 대략(大略) 다음과 같다. (1) 본균(本菌)의 최적작용(最適作用) pH는 7.2부근(附近)에 위치(位置)하며 (2) 최적작용온도(最適作用溫度)에 있어서는 추출효소(抽出酵素)나 건조균체(乾燥菌體)를 그대로 사용(使用)하거나 다같이 $75^{\circ}C$부근(附近)에 위치(位置) 하며. (3) 본(本) 효소(酪素)는 그 작용(作用)에 있어서 기질(基質)인 Glucose의 농도(濃度)가 포화상태(飽和狀態)에서도 조해(阻害) 현상(現象)을 나타내지 않으며 (4) 본(本) 효소(酵素)의 Glucose에 대(對)한 최고(最高) 이성화율(異性化率)은 약(約) 50%에 달(達)하며 반응조건(反應條件)을 개선(改善)하므로서 더 높은 이성화율(異性化率)을 얻을수 있으리라고 생각되며 (5) 본(本) 효소(酵素)는 $80^{\circ}C$의 온도(溫度)에서는 거의 대부분(大部分)이 실활(失活)됨을 알 수 있었다.

Some properties of glucose isomerizing enzyme which produced by the strain K-17 in xylose containing nutrient broth medium were investigated. The optimal pH for enzyme reaction was indicated about 7.2 and optimal temperature was about $75^{\circ}C$. The same optimal temprature was indicated by both cell free extract and acetone dried cells using as enzyme. The glucose isomerizing enzyme from strain K-17 was not inhibited by the high concentration of substrate even in a suturated glucose solution, but most enzyme was inactivated by the heat treatment at $80^{\circ}C$. The maximum fructose forming ratio from glucose was about 50 percents.

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